5777

This invention is a microelectrochemical sensing device that detects the agent of chronic wasting disease. Chronic wasting disease is a prion disease, caused by a misfolded prion protein (PrPSc). Mammals normally express the healthy prion protein (PrPC). The disease associated form (PrPSc) has the same nucleic acid sequence - making accurate diagnoses challenging. Current diagnostic modalities take hours or days to get test results, are expensive to run, are laborious, and require expertise to conduct.

 

This biosensor is a rapid sensor that detects PrPC and PrPSc in an outstanding 3-minute timeframe. The sensor is currently capable of discriminating PrPSc from PrPC at sensitivities just below a commercially available enzyme immunoassay. Ongoing optimization of the biosensor is expected to further increase performance relative to conventionally utilized/approved methods.

• Precise, fast, and low-cost machine-learning-assisted microelectrochemical biosensing device for quantification of CWD prions in brain tissue samples from deer.

• Machine-learning-assisted electrochemical biosensor for detecting misfolded prion protein in real samples of deer.

• This device will facilitate effective management interventions for prognosis of deer with CWD.

Stakeholders would include state wildlife agencies, state diagnostic laboratories, and National Veterinary Services Laboratories.

Patent(s) applied for