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Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique and has been widely used for detection of pathogens in many organisms. Current LAMP-based sensors usually require the LAMP products to be labeled in order for them to be detected.

 

This new LAMP chip offers a new portable platform to detect pathogens using label-free sensors with ultrasensitivity ∼1 fg/μL (1.6 × 10–6 attomole/μL) and high specificity in a short period of time (∼30 min), indicating its suitability for point-of-care testing and on-site diagnostics. Unlike the currently available LAMP-based sensors, which usually require labeling of LAMP amplicons that adds additional time, cost, and complexity, the label-free LAMP chip is capable of directly detecting LAMP products bound to the sensors.
Furthermore, this LAMP chip provides a readily adaptable and generic technical platform for detecting a variety of nucleic acid products. It can be operated in combination with RT-LAMP and RCA for the detection of both DNA and RNA molecules. Therefore, the label-free LAMP chip platform is ultrasensitive, highly specific, rapid, and cost-effective and can be broadly applied to detect a wide range of diseases in plants, animals, and humans.

• Readily adaptable and generic technical platform for detecting a variety of nucleic acid products

• Label-free sensor

• Ultrasensitive (∼1 fg/μL (1.6 × 10−6 attomole/μL)

• High specificity in a short period of time

• Portable

This technology can be broadly applied to detect a wide range of diseases in
plants, animals, and humans

1. Subin Mao, Jinping Zhao, Xiaoke Ding, Van Anh Vuong, Junqi Song, Long Que, "Integrated Sensing Chip for Ultrasensitive Label-Free Detection of the Products of Loop-Mediated Isothermal Amplification", American Chemical Society, 2023, 8, 6, 2255-2262

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