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A paper-based assay for quntitation and identification of glycan and oligosaccharide sugars
Category(s):
For Information, Contact:
Mark Juetten
Associate Commercialization Manager, Chemistry
515-294-4740
licensing@iastate.edu
Web Published:
10/8/2018
ISURF #
4663
Summary:
ISU researchers have developed a colometric paper assay, able to rapidly detect glycan linkages.

Development Stage:
Description:
Viable synthetic human milk relies on development new methods of making the human milk olgiosaccharides (HMO) that are necessary for supplementing formula. New synthesis methods will require monitoring and analysis of the glycan (oligiosaccharide) products. Current analysis of glycans is limited in throughput and scope by the need for LC/MS technology, which can take roughly an hour for one sample, and requires expensive equipment, specialized columns and derivatizing agents, and expertise on the operation of the equipment. Higher throughput detection would make synthesis of glycans more viable via combinatorial chemical synthesis or metabolic engineering, both of which require the testing of many samples. Paper is a common material for low-cost point-of-use diagnostic where rapid throughput is desirable. A paper based glycan detection kit would therefore have utility for rapid detection for aiding glycan synthesis. Iowa State University researchers have developed a paper based assay that allows for colorimetric detection of glycans for rapid detection and quantification. The assay has enzymes imprinted onto the paper strips that selectively produce a color change when exposed to specific oligiosaccharides linkages. The assay is quantifiable and can be completed in 5 minutes. The assay can be applied to high-throughput HMO detection.

Advantage:
• Low cost
• Rapid feedback
• High throughput

Application:
Glycan (oligiosaccharide) detection, glycan synthesis
Patent Information:
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Patent:
Patent(s) applied for

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