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Optical Nanosensors for Hydrolytic Enzyme Characterization
Category(s):
For Information, Contact:
Mark Juetten
Associate Commercialization Manager, Chemistry
515-294-4740
licensing@iastate.edu
Web Published:
2/9/2018
ISURF #
4667
Summary:
Iowa State University Researchers have developed a new biosensor for hydrolytic enzyme characterization based on single walled carbon nanotubes.

Development Stage:
Description:
Single walled carbon nanotubes (SWNT) are becoming ubiquitous in chemical sensing applications. Able to fluoresce in the near-IR, with no photo-bleaching threshold, they are ideal for prolonged imaging in living cells and tissues. Although SWNT are usually insoluble in water, Iowa State University researches wrapped them in amphiphilic polymers, thereby solubilizing them. By destroying or altering this polymer wrapping, the SWNTs collapse into their insoluble aggregate form and the fluorescence is quenched. When the SWNT is “wrapped” with moieties susceptible to cleavage via hydrolytic enzymes, this turn-off character can be used to quantify enzyme activity. This tool can be used for high-throughput screeening of enzyme specificity, engineered activity optimization and dependence on solution conditions (temperature, pH, salts, ect.). ISU researches have demonstrated the efficacy of the technique for a number of types of enzymes, measuring their turnover with high precision.

Advantage:
• Stable in a range of media and pHs
• High tolerance to photobleaching effects
• Fast responses
• Highly sensitive

Application:
Hydrolytic enzyme characterization
Patent Information:
*To see the full version of the patent(s), follow the link below, then click on "Images" button.

Patent:
Patent(s) applied for

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