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PDCoV S1 Protein ELISA for Detection of Porcine Delta Coronavirus
Category(s):
For Information, Contact:
Dario Valenzuela
Senior Commercialization Manager, Life Sciences
515-294-4740
licensing@iastate.edu
Web Published:
12/8/2015
ISURF #
4296
Summary:
Researchers at Iowa State University have developed an ELISA assay for the detection of Porcine Delta Coronavirus based on the use of a recombinant S1 viral protein.

Development Stage:
Description:
Porcine Delta Coronavirus (PDCoV) was first diagnosed in North America in early 2014 where it was associated with enteric disease in pigs. While assays detecting nucleic acids of the virus were implemented quickly, assays to detect anti-PDCoV antibodies lagged far behind. Iowa State Researchers led the development of an indirect PDCoV anti-IgG enzyme-linked immunosorbent assay (ELISA) based on the putative S1 portion of the spike protein. The assay has been validated and was utilized to determine the prevalence of PDCoV in U.S. pigs. The diagnostic sensitivity of the PDCoV ELISA was 91% with a diagnostic specificity of 95%. ISURF is making the materials available under a non-exclusive license.

Advantage:
• S1 protein has been used to validate PDCoV detection
• Detects IgG antibodies
• S1 polypeptide antigen highly conserved for broad detection

Application:
Veterinary Diagnostics

References:
1. PLoS One. 2015; 10(4): e0124363. Published online 2015 Apr 16. doi:  10.1371/journal.pone.0124363  PMCID: PMC4399883

2. Development and Application of an ELISA for the Detection of Porcine Deltacoronavirus IgG Antibodies, Anil Thachil,1 Priscilla F. Gerber,2 Chao-Ting Xiao,1 Yao-Wei Huang,3 and Tanja Opriessnig1,2,*

Patent Information:
*To see the full version of the patent(s), follow the link below, then click on "Images" button.

Intellectual Property:
Tangible Material

Direct Link: