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Researchers at Iowa State University have developed an ELISA assay for the detection of Porcine Epidemic Diarrhea Virus (PEDV) based on the use of the PEDV S1 protein. The assay is capable of detecting anti-PEDV antibody of various classes (IgG, IgA or IgM) in serum, oral fluid, feces, and in colostrum and milk.

Laboratory diagnosis for PEDV is often performed using PCR as PEDV is difficult to culture; in addition, PEDV can resemble transmissible gastroenteritis (TGE) clinically, so it is important for herd management to understand the cause of acute diarrhea in infected pigs. Other methods used for diagnosis include clinical signs, history, ELISA, examination of feces for virus using electron microscopy, and post-mortem examination. This technology circumvents many of the difficulties and time constraints associated with these traditional methods. ISURF is making the materials available under a non-exclusive license.

• Works with a number of biological samples

• Detects various classes of Ig

• Can be used with a variety of sample types

Veterinary Diagnostics

Tangible Material