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Hybridoma Cell Lines for Mycoplasma bovis
Category(s):
For Information, Contact:
Dario Valenzuela
Senior Commercialization Manager, Life Sciences
515-294-4740
licensing@iastate.edu
Web Published:
5/22/2015
ISURF #
1908
Description:
Two mouse/mouse stable hybridomas (87, 163) have been developed against M. bovis strain M23. M. bovis is associated with a variety of bovine diseases including arthritis and pneumonia of calves and young cattle, mastitis, and genital tract infections. It is considered the most virulent bovine mycoplasma in the US. Ascite fluid from these two hybridoma cells can be use in formalin-fixed, paraffin-embedded tissues and is highly specific in ELISA, IFA, and immunoblot analyses to six strains of M. bovis (M23, Emm, Bat, 1315, M66, and M84) originating from cases of bovine pneumonia or mastitis. They show no cross-reactivity with other bovine mycoplasma species often encountered in the US, Canada, and Europe. These species include M. dispar (SDO), M. alkalescens (PG31), M. bovirhinus (CS260), M. arginini (630), M. bovuculi (FS8-7), M. bovigenitalium (ATCC 14173), M. verecundum (ATCC 27862), andM. canadense (275C). Acite fluid from MYB 87 reacts with M. agalactiae (GM 139), a rare sheep and goat pathogen highly related to M. bovis, in IFA tests but not in ELISA. Ascite fluid from MYB 163 does not react with M. agalactiae. MYB 63 is of IgG1 Kappa isotype, while MYB 87 is of IgM Kappa isotype. These hybridomas should be useful in the diagnosis and detection of M. bovis and its related diseases.
Patent Information:
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