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Universal and Differential Serologic Assay for Influenza A Virus
Category(s):
For Information, Contact:
Dario Valenzuela
Senior Commercialization Manager, Life Sciences
515-294-4740
licensing@iastate.edu
Web Published:
5/13/2015
ISURF #
3288
Summary:
ISU researchers have developed a diagnostic assay for swine influenza (Influenza A) that can distinguish between exposed and vaccinated animals. This ELISA-based assay is also able to detect all Influenza A viruses, regardless of subtype. The assay represents an improvement over currently available tests, and may help pork producers in managing this costly disease.

Development Stage:
The serologic assay has been used to characterize the antibody responses of pigs to SIV antigens, and is ready for additional field evaluation.

Description:
Swine influenza, or Influenza A, is an acute respiratory disease that can affect pigs of all ages and results in significant economic losses for pork producers. While detection of swine influenza virus (SIV) or viral antigen in the lung or nasal secretions from symptomatic animals is considered the definitive diagnosis of swine influenza, serologic tests are often used to detect animals that have been exposed to the virus since the disease has a very short duration and the virus quickly becomes undetectable. However, interpretation of serologic test results for the diagnosis of SIV is made difficult by the intensive use of vaccination, and the emergence of new subtypes and antigenic drift within a subtype. To address the need for serologic assays for SIV that detect infection regardless of subtype or antigenic variation, as well as the need to differentiate exposed from vaccinated animals, ISU researchers have developed a universal and differential serologic assay for influenza A virus. This ELISA-based assay detects two antigens: one that is conserved among all influenza A viruses, and a second antigen that is expressed in exposed but not vaccinated pigs.

Advantage:
• Comprehensive (the assay detects an antigen conserved among all swine influenza virus subtypes)
• Differential (can distinguish between exposed and vaccinated animals)
• Stable (use of recombinant antigens enables continuous production of reagents)
Application:
Diagnosis of swine influenza (Influenza A)

References:
Patent Information:
*To see the full version of the patent(s), follow the link below, then click on "Images" button.
Country Serial No. Patent No. Issued Date
United States 11/473,434 7,892,729* 2/22/2011


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